User:Michael F. Nagle/Notebook/Chem 571/2012/10/23: Difference between revisions
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*Analyze 60-170 [Au/BSA] samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|yesterday]] via atomic absorbance spectroscopy to determine concentration of AuNPs in solution | *Analyze 60-170 [Au/BSA] samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|yesterday]] via atomic absorbance spectroscopy to determine concentration of AuNPs in solution | ||
==Starter and Expression Culturess== | ==Starter and Expression Culturess== | ||
* The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA) | * The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA) | ||
* Samples of Au/BSA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]] were analyzed by Atomic Absorbtion Spectroscopy. | * Samples of Au/BSA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]] were analyzed by Atomic Absorbtion Spectroscopy. | ||
**Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing. | **Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing. | ||
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[[Image:AAgold2.jpg]] | [[Image:AAgold2.jpg]] | ||
==Discussion== | ==Discussion== | ||
*Our AA spectra is very similar to the one obtained [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]] for 60-170 | *Our AA spectra is very similar to the one obtained [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]] for 60-170 [Au/BSA]. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error. | ||
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Revision as of 02:29, 7 December 2012
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Starter and Expression Culturess
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