User:Michael F. Nagle/Notebook/Chem 571/2012/10/23

Objectives

• Express ADA with E. Coli
  • ADA will be used to nucleate AuNPs
• Analyze 60-170 [Au/BSA] samples prepared yesterday via atomic absorbance spectroscopy to determine concentration of AuNPs in solution

Starter and Expression Culturess

• The Expression Culture and Starter Culture procedures were followed with the same modifications as on[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more E. Coli, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)
• Samples of Au/BSA made last week were analyzed by Atomic Absorbtion Spectroscopy.
  • Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.
  • Between samples, water was run in order to clear residue.

Data

Discussion

• Our AA spectra is very similar to the one obtained 9/4 for 60-170 Au/BSA. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error.