User:Michael F. Nagle/Notebook/Chem 571/2012/10/23

(Difference between revisions)

Revision as of 05:28, 7 December 2012

Project name

Express ADA with E. Coli
- ADA will be used to nucleate AuNPs
Analyze 60-170 [Au/BSA] samples prepared yesterday via atomic absorbance spectroscopy to determine concentration of AuNPs in solution

The Expression Culture and Starter Culture procedures were followed with the same modifications as on 9/18. These cultures are to be used to grow more E. Coli, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)
Samples of Au/BSA made last week were analyzed by Atomic Absorbtion Spectroscopy.
- Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.
- Between samples, water was run in order to clear residue.

Our AA spectra is very similar to the one obtained 9/4 for 60-170 Au/BSA. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error.

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 *Analyze 60-170 [Au/BSA] samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|yesterday]] via atomic absorbance spectroscopy to determine concentration of AuNPs in solution
 ==Starter and Expression Culturess==
-* The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)
+* The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)
 * Samples of Au/BSA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]] were analyzed by Atomic Absorbtion Spectroscopy.
 **Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.