User:Michael F. Nagle/Notebook/Chem 571/2012/10/17: Difference between revisions
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==Objectives== | |||
*Compare molar mass of plasmids via electrophoresis | |||
*Prepare new sets of 60-170 [Au/BSA] and [Au/Lysozyme] | |||
*Analyze chemiluminescence of HRP assay with luminol using modified UV/Vis instrument | |||
==Electrophoresis== | ==Electrophoresis== | ||
*5 μL of the plasmid mutated last week was placed in an autoclaved microcentrifuge tube. | *5 μL of the plasmid mutated last week was placed in an autoclaved microcentrifuge tube. | ||
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==Nucleation of AuNPs with BSA and Lysozyme== | ==Nucleation of AuNPs with BSA and Lysozyme== | ||
*[[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/17|Mary Mendoza]] prepared lysozyme and gold stock solutions. | *[[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/17|Mary Mendoza]] prepared lysozyme and gold stock solutions. | ||
* Solutions for both BSA and lysozyme and HAuCl<sub>4</sub> were prepared at the same mole ratios that [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|have previously been used with BSA]]. Since the same BSA stock was used, and the lysozyme stock was prepared at the same concentration, the volumes added to each tube did not change. | * Solutions for both BSA and lysozyme and HAuCl<sub>4</sub> were both prepared at the same mole ratios that [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|have previously been used with BSA]]. Since the same BSA stock was used, and the lysozyme stock was prepared at the same concentration, the volumes added to each tube did not change. | ||
**Both sets of solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours. | **Both sets of solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours. | ||
==Results and Discussion== | |||
*Besides the DNA ladder, nothing moved down the agarose gel in electrophoresis. This may be due to a problem with the samples, so they should be recreated before attempting electrophoresis again. | |||
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Revision as of 01:23, 7 December 2012
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Objectives
Electrophoresis
Chemiluminescence
Nucleation of AuNPs with BSA and Lysozyme
Results and Discussion
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