User:Michael F. Nagle/Notebook/Chem 571/2012/10/16: Difference between revisions
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==Procedure== | ==Procedure== | ||
* The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed | |||
** The specifications of the primer used are as follows: | |||
[[Image:Primer212.png]] | |||
**1mL molecular biology grade water was added to the primer. | |||
**The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated | |||
Target concentration: 100ng/uL | |||
***0.49mg/1000μL = 490ng/μL | |||
***100ng/uL*1000uL / 490ng/uL = 204.08μL | |||
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Revision as of 23:38, 6 December 2012
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Procedure
Target concentration: 100ng/uL
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