User:Michael F. Nagle/Notebook/Chem 571/2012/10/16: Difference between revisions
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**[[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/10/16|Keyun Wang]] prepared 100ng/uL solution of the matching forward primer. | **[[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/10/16|Keyun Wang]] prepared 100ng/uL solution of the matching forward primer. | ||
**Solution was prepared with the following: | **Solution was prepared with the following: | ||
***1uL forward primer ***1uL reverse primer ***1uL wild strand ADA ***5uL 10x Pfu buffer ***40.6uL molecular biology grade water ***1uL Turbo DNA polymerase solution ***0.4Turbo DNA polymerase 25mM dNTPs | ***1uL forward primer | ||
***1uL reverse primer | |||
***1uL wild strand ADA | |||
***5uL 10x Pfu buffer | |||
***40.6uL molecular biology grade water | |||
***1uL Turbo DNA polymerase solution | |||
***0.4Turbo DNA polymerase 25mM dNTPs | |||
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Revision as of 23:46, 6 December 2012
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Procedure
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