User:Michael F. Nagle/Notebook/Chem 571/2012/10/16

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedure)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Procedure==
==Procedure==
-
# The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed  
+
* The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed  
-
# The specifications of the primer used are as follows:
+
** The specifications of the primer used are as follows:
-
 
+
[[Image:Primer212.png]]
 +
**1mL molecular biology grade water was added to the primer.
 +
**The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated
 +
Target concentration: 100ng/uL
 +
***0.49mg/1000μL = 490ng/μL
 +
***100ng/uL*1000uL / 490ng/uL = 204.08μL
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 02:38, 7 December 2012

Project name Main project page
Previous entry      Next entry

Procedure

  • The procedure for PCR Mutation was followed
    • The specifications of the primer used are as follows:

Image:Primer212.png

    • 1mL molecular biology grade water was added to the primer.
    • The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated
Target concentration: 100ng/uL
      • 0.49mg/1000μL = 490ng/μL
      • 100ng/uL*1000uL / 490ng/uL = 204.08μL


Personal tools