User:Michael F. Nagle/Notebook/Chem 571/2012/10/02: Difference between revisions
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#A .2M buffer of sodium phosphate was made at a pH of 7. | #A .2M buffer of sodium phosphate was made at a pH of 7. | ||
#.0017M hydrogen peroxide solution was made by adding 1mL 30% hydrogen peroxide to 100mL distilled water. 1mL of hydrogen peroxide solution was diluted to 50mL with 49mL of sodium phosphate buffer for 34μM solution. | #.0017M hydrogen peroxide solution was made by adding 1mL 30% hydrogen peroxide to 100mL distilled water. 1mL of hydrogen peroxide solution was diluted to 50mL with 49mL of sodium phosphate buffer for 34μM solution. | ||
#698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine solution was prepared with the sodium phosphate buffer. | #698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine (AAP) solution was prepared with the sodium phosphate buffer. | ||
#From the stocks, solution was prepared with 18mM iodophenol, 7.285μM AAP, 1.7mM H<sub>2</sub>O<sub>2</sub> and 115nM HRP. | |||
##Such a solution was analyzed for the 1st, 3rd and 4th runs. | |||
##For the 2nd run, HRP was increased to 2.3μM while AAP was kept at 7.285μM | |||
##HRP was increased to 50μM and AAP to 156.25 μM for the final trial. | |||
==Data== | |||
[[Image:Hrp1.png]] | |||
==Discussion== | ==Discussion== |
Revision as of 00:22, 7 December 2012
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Objective
Procedure
DataDiscussion
References1. Horseradish Peroxidase Assay. Gold Biotechnology File:3760-Protocol HRP AbsorbanceAssay.pdf |