User:Michael F. Nagle/Notebook/Chem 571/2012/10/02: Difference between revisions
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== | ==Objective== | ||
#Prepare reagents for HRP assay and complete HRP assay with and without gold nanoparticles to see how they affect the reaction. | |||
==Procedure== | |||
#1mL horseradish peroxidase was dissolved in 1mL distilled water. | |||
#A .2M buffer of sodium phosphate was made at a pH of 7. | |||
#.0017M hydrogen peroxide solution was made by adding 1mL 30% hydrogen peroxide to 100mL distilled water. 1mL of hydrogen peroxide solution was diluted to 50mL with 49mL of sodium phosphate buffer for 34μM solution. | |||
#698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine solution was prepared with the sodium phosphate buffer. | |||
==Discussion== | |||
#The HRP assays were not completed today, but will be [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/02]], with the reagents prepared today | |||
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Revision as of 21:39, 4 October 2012
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