User:Michael F. Nagle/Notebook/Chem 571/2012/09/26: Difference between revisions
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##The collumn was eluted with the binding buffer, then eluted with elution buffer, and again with the binding buffer to wash out excess imidazole. | ##The collumn was eluted with the binding buffer, then eluted with elution buffer, and again with the binding buffer to wash out excess imidazole. | ||
#ADA was inserted and eluted with the binding buffer. The collumn was eluted with the eluting buffer, which contains imidazole, because it competes for bonds with the nickel, forcing off the ADA. The ADA and protein were collected in 11 5mL tubes. | #ADA was inserted and eluted with the binding buffer. The collumn was eluted with the eluting buffer, which contains imidazole, because it competes for bonds with the nickel, forcing off the ADA. The ADA and protein were collected in 11 5mL tubes. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 11:45, 7 October 2012 (EDT)''':more details. what is the binding and elution buffers? flow rates? column size? what details are needed to repeat this experiment exactly? what did you do with the protein? | |||
==Data== | ==Data== |
Revision as of 08:45, 7 October 2012
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Objectives
Procedure
DataThese absorbance peaks show the amount of ADA in each tube. Discussion
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