User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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==Procedure== | ==Procedure== | ||
#Expressing ADA | #Expressing ADA | ||
## [[AU_Biomaterials_Design_Lab:Protocols/Protein_Expression]] | ## Procedure for protein expression was followed with noted changes, and can be found here: [[AU_Biomaterials_Design_Lab:Protocols/Protein_Expression]] | ||
## Tris stock for 1M solution | ## Tris stock for 1M solution | ||
Starter cultures were centrifuged for 15 minutes at 4500rpm and resuspended in 4mL LB broth. | Starter cultures were centrifuged for 15 minutes at 4500rpm and resuspended in 4mL LB broth. |
Revision as of 14:46, 6 October 2012
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Objectives
Procedure
Starter cultures were centrifuged for 15 minutes at 4500rpm and resuspended in 4mL LB broth.
ObservationsOne of the flasks with broth appeared a different shade of yellow this morning prior to centrifuging. |