User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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*begin protein expression | *begin protein expression | ||
*make elution and binding buffers to be used for extraction and purification of ADA | *make elution and binding buffers to be used for extraction and purification of ADA | ||
*prepare solutions of tris in Au/BSA, to be analyzed by UV/Vis to determine how various concentrations affect AuNPs in solution | |||
==Procedure== | ==Procedure== | ||
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** Cultures were prepared and E. Coli with a plasmid for Adenosine Deaminase (ADA) were added and incubated overnight, by [[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/09/19|Mary Mendoza]]. She also prepared binding and elution buffers for extraction. | ** Cultures were prepared and E. Coli with a plasmid for Adenosine Deaminase (ADA) were added and incubated overnight, by [[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/09/19|Mary Mendoza]]. She also prepared binding and elution buffers for extraction. | ||
**Binding and elution buffers were prepared by [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/19|Puja Mody]] | **Binding and elution buffers were prepared by [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/19|Puja Mody]] | ||
*In order to determine how various concentrations of tris affect AuNPs in solution, tris was added to 70 [HAuCl<sub>4</sub>/BSA] solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/12|last week]] at .05mM, .5mM, 5mM, 50mM, 100mM, 200mM, 500mM and 1M. These [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/25|are to be analyzed by UV/Vis]]. | *Tris added to Au/BSA | ||
*The following calculations were completed to determine how much tris was needed in each solution. [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/19|Puja Mody]] prepared the tris stock. | **In order to determine how various concentrations of tris affect AuNPs in solution, tris was added to 70 [HAuCl<sub>4</sub>/BSA] solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/12|last week]] at .05mM, .5mM, 5mM, 50mM, 100mM, 200mM, 500mM and 1M. These [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/25|are to be analyzed by UV/Vis]]. | ||
**The following calculations were completed to determine how much tris was needed in each solution. [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/19|Puja Mody]] prepared the tris stock. | |||
**m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | **m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ||
**1000mM*6mL=1000mM*x | **1000mM*6mL=1000mM*x | ||
Revision as of 21:31, 6 December 2012
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