User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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==Procedure== | ==Procedure== | ||
*Expressing ADA | |||
** Cultures were prepared and E. Coli with a plasmid for Adenosine Deaminase (ADA) were added and incubated overnight, by [[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/09/19|Mary Mendoza]]. She also prepared binding and elution buffers for extraction. | |||
**Binding and elution buffers were prepared by [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/19|Puja Mody]] | |||
*In order to determine how various concentrations of tris affect AuNPs in solution, tris solution was added to 70 [HAuCl<sub>4</sub>/BSA] solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/12|last week]] at .05mM, .5mM, 5mM, 50mM, 100mM, 200mM, 500mM and 1M. They are to be analyzed by UV/Vis. | |||
*The following calculations were completed to determine how much tris was needed in each solution. [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/19|Puja Mody]] prepared the tris stock. | |||
##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ||
##500mM*6mL=1000mM*x8##3mL | ##500mM*6mL=1000mM*x8##3mL |
Revision as of 21:26, 6 December 2012
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Objectives
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