User:Michael F. Nagle/Notebook/Chem 571/2012/09/18: Difference between revisions
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* Grow E. Coli in [[AU Biomaterials Design Lab:Materials/LB|LB]] broth, for the purpose of expressing ADA. | * Grow E. Coli in [[AU Biomaterials Design Lab:Materials/LB|LB]] broth, for the purpose of expressing ADA. | ||
==Procedure== | ==Procedure== | ||
# In four 2.8L flasks, broth was created with 25g [[AU Biomaterials Design Lab:Materials/LB|LB]] and 1L H<sub>2</sub>O. Four more smaller 250mL flasks were prepared using this protocol for making [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] | # In four 2.8L flasks, broth was created with 25g [[AU Biomaterials Design Lab:Materials/LB|LB]] and 1L H<sub>2</sub>O, using the protocol to make [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture]]. Four more smaller 250mL flasks were prepared using this protocol for making [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] | ||
# The starter culture was steamed in the autoclave following [[AU Biomaterials Design Lab:Protocols/Autoclave|this protocol]] | # The starter culture was steamed in the autoclave following [[AU Biomaterials Design Lab:Protocols/Autoclave|this protocol]] | ||
Revision as of 10:39, 19 September 2012
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