User:Michael F. Nagle/Notebook/Chem 571/2012/09/18

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedure)
Line 9: Line 9:
* Grow E. Coli in [[AU Biomaterials Design Lab:Materials/LB|LB]] broth, for the purpose of expressing ADA.
* Grow E. Coli in [[AU Biomaterials Design Lab:Materials/LB|LB]] broth, for the purpose of expressing ADA.
==Procedure==
==Procedure==
-
# In four 2.8L flasks, broth was created with 25g [[AU Biomaterials Design Lab:Materials/LB|LB]] and 1L H<sub>2</sub>O. Four more smaller 250mL flasks were prepared using this protocol for making [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]]
+
# In four 2.8L flasks, broth was created with 25g [[AU Biomaterials Design Lab:Materials/LB|LB]] and 1L H<sub>2</sub>O, using the protocol to make [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture]]. Four more smaller 250mL flasks were prepared using this protocol for making [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]]
# The starter culture was steamed in the autoclave following [[AU Biomaterials Design Lab:Protocols/Autoclave|this protocol]]
# The starter culture was steamed in the autoclave following [[AU Biomaterials Design Lab:Protocols/Autoclave|this protocol]]

Revision as of 12:39, 19 September 2012

Project name Main project page
Previous entry      Next entry

Objectives

  • Grow E. Coli in LB broth, for the purpose of expressing ADA.

Procedure

  1. In four 2.8L flasks, broth was created with 25g LB and 1L H2O, using the protocol to make expression culture. Four more smaller 250mL flasks were prepared using this protocol for making Starter Culture
  2. The starter culture was steamed in the autoclave following this protocol



Personal tools