User:Michael F. Nagle/Notebook/Chem 571/2012/09/18
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| - | == | + | ==Objectives== |
| - | * | + | * Grow E. Coli in [[AU Biomaterials Design Lab:Materials/LB|LB]] broth, for the purpose of expressing ADA. |
| + | ==Procedure== | ||
| + | # In four 2.8L flasks, broth was created with 25g [[AU Biomaterials Design Lab:Materials/LB|LB]] and 1L H<sub>2</sub>O. Four more smaller 250mL flasks were prepared using this protocol for making [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] | ||
| + | # The starter culture was steamed in the autoclave following [[AU Biomaterials Design Lab:Protocols/Autoclave|this protocol]] | ||
Revision as of 14:10, 18 September 2012
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