User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions

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==Objectives==
==Objectives==
* With UV/Vis, measure absorbance of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and formation of gold nanoparticles from [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] samples with mole ratios ranging from 120-134. The samples were prepared last Wednesday, when two round of UV/Vis were run. The third round should show if the Tris has changed the dissolution of fibers.
* With UV/Vis, measure absorbance of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and formation of gold nanoparticles from [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] samples with mole ratios ranging from 120-134.
* Measure absorbancy of samples created [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|9/5/12]] with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples
* Measure absorbancy of samples created [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|9/5/12]] with Tris buffer at pH=8 and compare spectra to the one obtained last week for the same samples
* Prepare solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] at a mole ratio that results in unfolded fibers in solution
* Prepare Au/BSA fibers for resuspension at pH 10


==Procedure==
==Procedure==
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##The same microcuvette was used and cleaned between each spectra.
##The same microcuvette was used and cleaned between each spectra.


#Solution was made with a mole ratio of 170
#Solution was made with a mole ratio of 170 using the same stock solutions and procedure as the 170 [Au/BSA] solution prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|8/29]]
##2.4268M BSA solution was used
###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
### 24.266μM * (volume) = 1μM * 6mL
### .247mL BSA solution
## 2150μM HAuCl4 solution was used
### 2150μM * (volume) = 6mL *(1μM*170)
### .474mL HAuCl<sub><sub>4</sub></sub> solution
### 8 such solutions were made. The solutions went in the oven at 80<sup>o</sup>C for 4 hours.
### 8 such solutions were made. The solutions went in the oven at 80<sup>o</sup>C for 4 hours.


==Data==
==Data==
[[Image:Bsanarrow.png]]
[[Image:Data2.JPG]]
[[Image:Data2.JPG]]


==Discussion==
==Discussion==
*No significant change was observed in the spectra for Tris solutions, indicating that no concentration affected the dissolution of fibers at pH 8.
*Resuspension with tris: There was no change at 528nm, the wavelength of interest for AuNPs, relative to the background. However, the background decreases on the third trial.


*Absorbance peaks were an order of magnitude or two greater than the first set of HAuCl<sub>4</sub> and BSA solutions
*Au/BSA at various ratios: This time, the peak in absorbance at 528nm is at 130 rather than 128. This is interesting because in [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|last set], the peak was at 128 and abs. dropped to near zero at 130. Also, in the last set, abs. was an order of magnitude greater than this time.




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Objectives

  • With UV/Vis, measure absorbance of BSA and formation of gold nanoparticles from HAuCl4 samples with mole ratios ranging from 120-134.
  • Measure absorbancy of samples created 9/5/12 with Tris buffer at pH=8 and compare spectra to the one obtained last week for the same samples
  • Prepare Au/BSA fibers for resuspension at pH 10

Procedure

  1. UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
  2. UV/Vis was run on HAuCl4, BSA and Tris solutions. Spectra were compared to those obtained last week.
    1. The same microcuvette was used and cleaned between each spectra.
  1. Solution was made with a mole ratio of 170 using the same stock solutions and procedure as the 170 [Au/BSA] solution prepared 8/29
      1. 8 such solutions were made. The solutions went in the oven at 80oC for 4 hours.

Data

Discussion

  • Resuspension with tris: There was no change at 528nm, the wavelength of interest for AuNPs, relative to the background. However, the background decreases on the third trial.
  • Au/BSA at various ratios: This time, the peak in absorbance at 528nm is at 130 rather than 128. This is interesting because in [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|last set], the peak was at 128 and abs. dropped to near zero at 130. Also, in the last set, abs. was an order of magnitude greater than this time.
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