User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions

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==Data==
==Data==
[[Image:Image:Data2.JPG]]
[[Image:Data2.JPG]]


==Discussion==
==Discussion==

Revision as of 13:46, 24 September 2012

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Objectives

  • With UV/Vis, measure absorbance of BSA and formation of gold nanoparticles from HAuCl4 samples with mole ratios ranging from 120-134. The samples were prepared last Wednesday, when two round of UV/Vis were run. The third round should show if the Tris has changed the dissolution of fibers.
  • Measure absorbancy of samples created 9/5/12 with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples
  • Prepare solutions of HAuCl4 and BSA at a mole ratio that results in unfolded fibers in solution

Procedure

  1. UV/Vis was run on HAuCl4 and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134.
  2. UV/Vis was run on HAuCl4, BSA and Tris solutions. Spectra were compared to those obtained last week.
    1. The same microcuvette was used and cleaned between each spectra.
  1. Solution was made with a mole ratio of 170
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*170)
      2. .474mL HAuCl4 solution
      3. 8 such solutions were made. The solutions went in the oven at 80oC for 4 hours.

Data

Discussion

  • No significant change was observed in the spectra for Tris solutions, indicating that no concentration affected the dissolution of fibers at pH 8.
  • Absorbance peaks were an order of magnitude or two greater than the first set of HAuCl4 and BSA solutions
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