User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions
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==Procedure== | ==Procedure== | ||
#UV/Vis was run on HAuCl<sub>4</sub> and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134. | #UV/Vis was run on HAuCl<sub>4</sub> and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134. | ||
#UV/Vis was run on HAuCl<sub>4</sub>, BSA and Tris solutions. Spectra were compared to those obtained | #UV/Vis was run on HAuCl<sub>4</sub>, BSA and Tris solutions. Spectra were compared to those obtained [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|last week]]. | ||
##The same microcuvette was used and cleaned between each spectra. | |||
##The same | |||
#Solution was made with a mole ratio of 170 | #Solution was made with a mole ratio of 170 | ||
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### 2150μM * (volume) = 6mL *(1μM*170) | ### 2150μM * (volume) = 6mL *(1μM*170) | ||
### .474mL HAuCl<sub><sub>4</sub></sub> solution | ### .474mL HAuCl<sub><sub>4</sub></sub> solution | ||
### 8 such solutions were made. The solutions went in the oven at 80<sup>o</sup>C for 4 hours. | |||
==Data== | ==Data== | ||
[[Image:Chem-_Redone_AuNP-BSA.xlsx]]<br>[[Image:Tris_buffer_AuNP_UV-Vis_pH_8.xlsx]] | [[Image:Chem-_Redone_AuNP-BSA.xlsx]]<br>[[Image:Tris_buffer_AuNP_UV-Vis_pH_8.xlsx]] |
Revision as of 13:33, 24 September 2012
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Objectives
Procedure
DataFile:Chem- Redone AuNP-BSA.xlsx
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