User:Michael F. Nagle/Notebook/Chem 571/2012/09/11: Difference between revisions
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==Objectives== | ==Objectives== | ||
* | * With UV/Vis, measure absorbance of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] and formation of gold nanoparticles from [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] samples with mole ratios ranging from 120-134. | ||
* | * Measure absorbancy of samples created [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|9/5/12]] with Tris buffer at pH=8 and compare spectra to those obtained last week for the same samples | ||
* Prepare solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] at a mole ratio that results in unfolded fibers in solution | |||
==Procedure== | |||
#UV/Vis was run on HAuCl<sub>4</sub> and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134. | #UV/Vis was run on HAuCl<sub>4</sub> and BSA solution with mole ratios of 120, 128, 130, 132, 133 and 134. | ||
#UV/Vis was run on HAuCl<sub>4</sub>, BSA and Tris solutions. Spectra were compared to those obtained last week. | |||
##The same cuvette was used and cleaned between each spectra. | ##The same cuvette was used and cleaned between each spectra. | ||
#Solution was made with a mole ratio of 170 | #Solution was made with a mole ratio of 170 | ||
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### 2150μM * (volume) = 6mL *(1μM*170) | ### 2150μM * (volume) = 6mL *(1μM*170) | ||
### .474mL HAuCl<sub><sub>4</sub></sub> solution | ### .474mL HAuCl<sub><sub>4</sub></sub> solution | ||
==Data== | |||
[[Image:Chem-_Redone_AuNP-BSA.xlsx|Au/BSA solutions with mole ratios from 120-134]] | |||
[[Image:Tris_buffer_AuNP_UV-Vis_pH_8.xlsx|Au/BSA solutions with Tris at pH=8]] | |||
Revision as of 21:25, 13 September 2012
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Objectives
Procedure
DataFile:Chem- Redone AuNP-BSA.xlsx File:Tris buffer AuNP UV-Vis pH 8.xlsx
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