User:Michael F. Nagle/Notebook/Chem 571/2012/09/05: Difference between revisions

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==Objectives==
==Objectives==
* Prepare new set of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] solutions exactly as the [[last set|User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29]], to be analyzed via UV/Vis
* Prepare new set of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] solutions exactly as the [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|last set]], to be analyzed via UV/Vis
* Via UV/Vis, determine optimal concentration of Tris at pH of 8 for resuspension of Au/BSA fibers
* Via UV/Vis, determine optimal concentration of Tris at pH of 8 for resuspension of Au/BSA fibers


Revision as of 20:34, 6 December 2012

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Objectives

  • Prepare new set of HAuCl4/BSA solutions exactly as the last set, to be analyzed via UV/Vis
  • Via UV/Vis, determine optimal concentration of Tris at pH of 8 for resuspension of Au/BSA fibers

Resuspension of Au/BSA Fibers with Tris buffer

  • UV/Vis was run twice on the samples prepared yesterday via serial dilution. Each spectra was obtained an hour apart.

Procedure

  • Solutions were made with the mole ratios of (HAuCl4/BSA) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. HAuCl4 and BSA Stock solutions made last week were used. From the stock, solutions of the same volume were made at the same mole ratios as before, so the procedure was exactly the same.
  • The tubes were wrapped in tin foil and heated at 80°C for 4 hours.

Data

Discussion

  • The fact that no change is seen at 528nm relative to background as mole ratios change, this indicates that Tris does not resuspend the fibers at pH 10 and the concentrations tested.


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