User:Michael F. Nagle/Notebook/Chem 571/2012/09/05: Difference between revisions

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==Data==
==Data==
*Since the samples with Tris buffer went through a third round of UV/Vis on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11#Data|9/11/12]], the spectra for all three are in the data section for that day.
*Since the samples with Tris buffer went through a third round of UV/Vis on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11#Data|9/11/12]], the spectra for all three are in the data section for that day and conclusions for the two days are combined.
 
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 14:33, 17 September 2012 (EDT)''':there is no indication that you collected absorbance spectra of anything on this page. what did you measure the absorbance of ? do you have any conclusions or observations for the work? 
 
 
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Revision as of 13:29, 24 September 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objectives

  • Make solutions with varying concentrations of Tris buffer and HAuCl4 and BSA fibers to find which concentrations are optimal for formation of fibers.
  • Find optimal mole ratio of HAuCl4 to BSA for formation of gold nanoparticles

Procedure

  1. Solutions were made with the mole ratios of (HAuCl4/BSA) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.


  1. Tris buffer serial dilution
    1. Serial dilution was done just as it was the day before
    2. 1mol Tris /1L = 1M
    3. 121.14g Tris
    4. 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris.
    5. 9mL H2O was put in the first tube to receive 1mL Tris from the stock solution.
    6. A serial dilution was repeated with H2O rather than Tris.
    1. UV/Vis was run twice on the samples that just received the serial dilution of Tris. Each spectra was obtained an hour apart.
  2. Au/BSA varying mole ratios
    1. A stock solution of HAuCl4 was made, with the attempted Molarity of 10mM.
      1. (moles HAuCl4)/.025L H2O = .01M
      2. 2.5*10-4 mol HAuCl4
      3. 2.5*10-4 mol * 339.785mol/g HAuCl4 = .0849g
      4. .0283g of the .0924g stuck to the weigh paper, leaving .0639gHAuCl4.
      5. .0639g HAuCl4 * (1molHAuCl4/339.79g/molHAuCl4)= xM HAuCl4
      6. .000188mol/.025L=xM HAuCl4
      7. .007520M HAuCl4
      8. .0639g HAuCl4 was put in 25mL to make a 10mM solution
    2. A 15μM stock solution of BSA was made
      1. (moles BSA)/.025L = .000015
      2. 3.75*10^-7mol BSA
      3. 3.75*10^-7mol BSA * 66,463g/mol BSA = .0249g BSA
      4. .0249g BSA was put in 25mL to make a 15μM solution
    3. Solutions were made with the mole ratios of (HAuCl4/BSA) 120, 128, 130, 132, 133, and 134. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.
      1. m1v1=m2v2
      2. 15μM*(volume BSA stock solution) = (1.5*6)
        1. 0.6mL BSA stock solution in each tube
      3. 7520μM*x mL=6mL*(1.5*120μM)
        1. 0.143mL HAuCl4 stock solution
        2. 5.257mL H2O
      4. 7520μM*x mL=6mL*(1.5*128μM)
        1. 0.153mL HAuCl4 stock solution
        2. 5.247mL H2O
      5. 7520μM*x mL=6mL*(1.5*130μM)
        1. .155mL HAuCl4 stock solution
        2. 5.245mL H2O
      6. 7520μM*x mL=6mL*(1.5*132μM)
        1. 0.157mL HAuCl4 stock solution
        2. 5.243mL H2O
      7. 7520μM*x mL=6mL*(1.5*133μM)
        1. 0.159mL HAuCl4 stock solution
        2. 5.241mL H2O
      8. 7520μM*x mL=6mL*(1.5*134μM)
        1. 0.160mL HAuCl4 stock solution
        2. 5.240mL H2O
  1. The tubes were wrapped in tin foil and heated at 80°C for 4 hours.
  • Abigail E. Miller 14:36, 17 September 2012 (EDT):explain what you number is. see note on previous entry.

Data

  • Since the samples with Tris buffer went through a third round of UV/Vis on 9/11/12, the spectra for all three are in the data section for that day and conclusions for the two days are combined.


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