User:Michael F. Nagle/Notebook/Chem 571/2012/09/05: Difference between revisions

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==Objectives==
==Objectives==
* Using UV/Vis, find which mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] result in the most [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] unfolding and formation of gold nanoparticles.
* Make solutions with varying concentrations of Tris buffer and [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
* Make solutions with varying concentrations of Tris buffer and [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
*Find optimal mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] for formation of gold nanoparticles
==Procedure==
==Procedure==
#UV/Vis
#Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL.
##1mL of each solution with a varying mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was inserted into a cuvette, which went into a UV/Vis Spectroscoper
##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
##Spectra were obtained and peaks for gold nanoparticles were identified at the 520nm range.
##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6)
##Cuvette was cleaned between each spectra, and the same cuvette was used each time
###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube
##7720μM*x mL=6mL*(1.5*60μM)
###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.331 mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*80μM)
###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.307mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*100μM)
###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.283mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*120μM)
###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.266mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*128μM)
###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.251mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*130μM)
###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.248mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*132μM)
###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.246mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*133μM)
###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.245mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*134μM)
###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.254mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*136μM)
###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.244mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*138μM)
###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.242mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*140μM)
###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.237mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*160μM)
###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.213mL H<sub>2</sub>O
##7720μM*x mL=6mL*(1.5*170μM)
###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
###5.202mL H<sub>2</sub>O
##The tubes were wrapped in tin foil and heated at 80°C for 4 hours.
 
#Tris buffer serial dilution
#Tris buffer serial dilution
## 1mol Tris /1L = 1M
## 1mol Tris /1L = 1M

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Objectives

  • Make solutions with varying concentrations of Tris buffer and HAuCl4 and BSA fibers
  • Find optimal mole ratio of HAuCl4 to BSA for formation of gold nanoparticles

Procedure

  1. Solutions were made with the mole ratios of (HAuCl4/BSA) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.
    1. m1v1=m2v2
    2. 15μM*(volume BSA stock solution) = (1.5*6)
      1. 0.6mL BSA stock solution in each tube
    3. 7720μM*x mL=6mL*(1.5*60μM)
      1. 0.069mL HAuCl4 stock solution
      2. 5.331 mL H2O
    4. 7720μM*x mL=6mL*(1.5*80μM)
      1. 0.093mL HAuCl4 stock solution
      2. 5.307mL H2O
    5. 7720μM*x mL=6mL*(1.5*100μM)
      1. 0.117mL HAuCl4 stock solution
      2. 5.283mL H2O
    6. 7720μM*x mL=6mL*(1.5*120μM)
      1. 0.134mL HAuCl4 stock solution
      2. 5.266mL H2O
    7. 7720μM*x mL=6mL*(1.5*128μM)
      1. 0.149mL HAuCl4 stock solution
      2. 5.251mL H2O
    8. 7720μM*x mL=6mL*(1.5*130μM)
      1. .152mL HAuCl4 stock solution
      2. 5.248mL H2O
    9. 7720μM*x mL=6mL*(1.5*132μM)
      1. 0.154mL HAuCl4 stock solution
      2. 5.246mL H2O
    10. 7720μM*x mL=6mL*(1.5*133μM)
      1. 0.155mL HAuCl4 stock solution
      2. 5.245mL H2O
    11. 7720μM*x mL=6mL*(1.5*134μM)
      1. 0.156mL HAuCl4 stock solution
      2. 5.254mL H2O
    12. 7720μM*x mL=6mL*(1.5*136μM)
      1. 0.159mL HAuCl4 stock solution
      2. 5.244mL H2O
    13. 7720μM*x mL=6mL*(1.5*138μM)
      1. 0.161mL HAuCl4 stock solution
      2. 5.242mL H2O
    14. 7720μM*x mL=6mL*(1.5*140μM)
      1. 0.163mL HAuCl4 stock solution
      2. 5.237mL H2O
    15. 7720μM*x mL=6mL*(1.5*160μM)
      1. 0.187mL HAuCl4 stock solution
      2. 5.213mL H2O
    16. 7720μM*x mL=6mL*(1.5*170μM)
      1. 0.198mL HAuCl4 stock solution
      2. 5.202mL H2O
    17. The tubes were wrapped in tin foil and heated at 80°C for 4 hours.
  1. Tris buffer serial dilution
    1. 1mol Tris /1L = 1M
    2. 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris.
    3. 9mL H2O was put in the first tube to receive 1mL Tris from the stock solution.
    4. A serial dilution was repeated with H2O rather than Tris.
    5. Test tubes were wrapped and heated at at 80°C for 4 hours.
  2. Au/BSA varying mole ratios
    1. A stock solution of HAuCl4 was made, with the attempted Molarity of 10mM.
      1. (moles HAuCl4)/.025L H2O = .01M
      2. 2.5*10-4 mol HAuCl4
      3. 2.5*10-4 mol * 339.785mol/g HAuCl4 = .0849g
      4. .0283g of the .0924g stuck to the weigh paper, leaving .0639gHAuCl4.
      5. .0639g HAuCl4 * (1molHAuCl4/339.79g/molHAuCl4)= xM HAuCl4
      6. .000188mol/.025L=xM HAuCl4
      7. .007520M HAuCl4
      8. .0639g HAuCl4 was put in 25mL to make a 10mM solution
    2. A 15μM stock solution of BSA was made
      1. (moles BSA)/.025L = .000015
      2. 3.75*10^-7mol BSA
      3. 3.75*10^-7mol BSA * 66,463g/mol BSA = .0249g BSA
      4. .0249g BSA was put in 25mL to make a 15μM solution
    3. Solutions were made with the mole ratios of (HAuCl4/BSA) 120, 128, 130, 132, 133, and 134. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.
      1. m1v1=m2v2
      2. 15μM*(volume BSA stock solution) = (1.5*6)
        1. 0.6mL BSA stock solution in each tube
      3. 7520μM*x mL=6mL*(1.5*120μM)
        1. 0.143mL HAuCl4 stock solution
        2. 5.257mL H2O
      4. 7520μM*x mL=6mL*(1.5*128μM)
        1. 0.153mL HAuCl4 stock solution
        2. 5.247mL H2O
      5. 7520μM*x mL=6mL*(1.5*130μM)
        1. .155mL HAuCl4 stock solution
        2. 5.245mL H2O
      6. 7520μM*x mL=6mL*(1.5*132μM)
        1. 0.157mL HAuCl4 stock solution
        2. 5.243mL H2O
      7. 7520μM*x mL=6mL*(1.5*133μM)
        1. 0.159mL HAuCl4 stock solution
        2. 5.241mL H2O
      8. 7520μM*x mL=6mL*(1.5*134μM)
        1. 0.160mL HAuCl4 stock solution
        2. 5.240mL H2O
  1. The tubes were wrapped in tin foil and heated at 80°C for 4 hours.


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