User:Michael F. Nagle/Notebook/Chem 571/2012/09/05: Difference between revisions
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##Cuvette was cleaned between each spectra, and the same cuvette was used each time | ##Cuvette was cleaned between each spectra, and the same cuvette was used each time | ||
#Tris buffer serial dilution | #Tris buffer serial dilution | ||
## | ## 1mol Tris /1L = 1M | ||
## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. | ## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. | ||
## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution. | ## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution. |
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