User:Michael F. Nagle/Notebook/Chem 571/2012/09/05: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
Line 18: | Line 15: | ||
##Cuvette was cleaned between each spectra, and the same cuvette was used each time | ##Cuvette was cleaned between each spectra, and the same cuvette was used each time | ||
#Tris buffer serial dilution | #Tris buffer serial dilution | ||
## | ## | ||
## | ## A partner did the serial dilution while I was doing UV/Vis and I appear to have copied a calculation down wrong because the henderson-hasselbach equation does not add up. | ||
## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. | ## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. | ||
## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution. | ## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution. | ||
## A serial dilution was repeated with H<sub>2</sub>O rather than Tris. | ## A serial dilution was repeated with H<sub>2</sub>O rather than Tris. | ||
## Test tubes were wrapped and heated at at 80°C for 4 hours. | ## Test tubes were wrapped and heated at at 80°C for 4 hours. | ||
#Au/BSA varying mole ratios | |||
##A stock solution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was made, with the attempted Molarity of 10mM. | |||
### (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M | |||
### 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
### 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g | |||
### .0283g of the .0924g stuck to the weigh paper, leaving .0639g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]. | |||
### .0639g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
### .000188mol/.025L=xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
### .007520M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | |||
### .0639g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution | |||
##A 15μM stock solution of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was made | |||
### (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 | |||
### 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] | |||
### 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] | |||
### .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution | |||
##Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 120, 128, 130, 132, 133, and 134. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL. | |||
###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
###15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) | |||
####0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | |||
###7520μM*x mL=6mL*(1.5*60μM) | |||
####0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.331 mL H<sub>2</sub>O | |||
###7520μM*x mL=6mL*(1.5*120μM) | |||
####0.143mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.257mL H<sub>2</sub>O | |||
###7520μM*x mL=6mL*(1.5*128μM) | |||
####0.153mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.247mL H<sub>2</sub>O | |||
###7520μM*x mL=6mL*(1.5*130μM) | |||
####.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.245mL H<sub>2</sub>O | |||
###7520μM*x mL=6mL*(1.5*132μM) | |||
####0.157mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.243mL H<sub>2</sub>O | |||
###7520μM*x mL=6mL*(1.5*133μM) | |||
####0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.241mL H<sub>2</sub>O | |||
###7520μM*x mL=6mL*(1.5*134μM) | |||
####0.160mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |||
####5.240mL H<sub>2</sub>O | |||
#The tubes were wrapped in tin foil and heated at 80°C for 4 hours. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 20:38, 10 September 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
Procedure
|