User:Michael F. Nagle/Notebook/Chem 571/2012/09/04: Difference between revisions
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==Procedure== | ==Procedure== | ||
#UV/Vis | #UV/Vis | ||
## | ##On [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|8.29]], samples were made with varying mole concentration of HAuCl<sub>4</sub> and BSA. .1mL of each solution was inserted into a cuvette, which went into a UV/Vis Spectroscoper | ||
##Cuvette was cleaned between each spectra, and the same cuvette was used each time | ##Cuvette was cleaned between each spectra, and the same cuvette was used each time | ||
#Tris buffer serial dilution | #Tris buffer serial dilution | ||
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#### HCl was added dropwise until the pH changed to 8 and 10 in each tube, as indicated by a pH meter. 9mL HCl was added for pH=8 and 2mL was used for ph=10. | #### HCl was added dropwise until the pH changed to 8 and 10 in each tube, as indicated by a pH meter. 9mL HCl was added for pH=8 and 2mL was used for ph=10. | ||
#### Sufficient water was added to leave the final volume of the solution at 100mL. | #### Sufficient water was added to leave the final volume of the solution at 100mL. | ||
## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution. | |||
## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. | ## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris. | ||
## A serial dilution was repeated with H<sub>2</sub>O rather than Tris. | ## A serial dilution was repeated with H<sub>2</sub>O rather than Tris. | ||
==Data== | ==Data== |
Revision as of 11:45, 18 September 2012
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