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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Objective== | | ==Objective== |
| *Find optimal mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] for formation of gold nanoparticles | | * Using UV/Vis, find which mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] result in the most [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] unfolding and formation of gold nanoparticles. |
| *Make Tris stock solutions and do serial dilution with [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers in Tris buffer | | *Make Tris stock solutions and do serial dilution with [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers in Tris buffer |
| ==Procedure== | | ==Procedure== |
| #Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL. | | #UV/Vis |
| ##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | | ##1mL of each solution with a varying mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was inserted into a cuvette, which went into a UV/Vis Spectroscoper |
| ##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6)
| | ##Spectra were obtained and peaks for gold nanoparticles were identified at the 520nm range. |
| ###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | | ##Cuvette was cleaned between each spectra, and the same cuvette was used each time |
| ##7720μM*x mL=6mL*(1.5*60μM)
| |
| ###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.331 mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*80μM)
| |
| ###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.307mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*100μM)
| |
| ###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.283mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*120μM)
| |
| ###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.266mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*128μM)
| |
| ###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.251mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*130μM)
| |
| ###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.248mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*132μM)
| |
| ###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.246mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*133μM)
| |
| ###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.245mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*134μM)
| |
| ###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.254mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*136μM)
| |
| ###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.244mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*138μM)
| |
| ###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.242mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*140μM)
| |
| ###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.237mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*160μM)
| |
| ###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.213mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*170μM)
| |
| ###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.202mL H<sub>2</sub>O
| |
| #The tubes were wrapped in tin foil and heated at 80°C for 4 hours.
| |
| #Tris buffer serial dilution | | #Tris buffer serial dilution |
| ## .1M solution of Tris was needed to begin serial dilutions. | | ## .1M solution of Tris was needed to begin serial dilutions. |
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