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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Objectives== | | ==Objective== |
| * Using UV/Vis, find which mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] result in the most [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] unfolding and formation of gold nanoparticles. | | *Find optimal mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] for formation of gold nanoparticles |
| * Make solutions with varying concentrations of Tris buffer and [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
| |
| ==Procedure== | | ==Procedure== |
| #UV/Vis | | #Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL. |
| ##1mL of each solution with a varying mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was inserted into a cuvette, which went into a UV/Vis Spectroscoper
| | ##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> |
| ##Spectra were obtained and peaks for gold nanoparticles were identified at the 520nm range.
| | ##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) |
| ##Cuvette was cleaned between each spectra, and the same cuvette was used each time
| | ###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube |
| #Tris buffer serial dilution
| | ##7720μM*x mL=6mL*(1.5*60μM) |
| ##
| | ###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ## A partner did the serial dilution while I was doing UV/Vis and I appear to have copied a calculation down wrong because the henderson-hasselbach equation does not add up.
| | ###5.331 mL H<sub>2</sub>O |
| ## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris.
| | ##7720μM*x mL=6mL*(1.5*80μM) |
| ## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution.
| | ###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ## A serial dilution was repeated with H<sub>2</sub>O rather than Tris.
| | ###5.307mL H<sub>2</sub>O |
| ## Test tubes were wrapped and heated at at 80°C for 4 hours.
| | ##7720μM*x mL=6mL*(1.5*100μM) |
| #Au/BSA varying mole ratios
| | ###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ##A stock solution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was made, with the attempted Molarity of 10mM.
| | ###5.283mL H<sub>2</sub>O |
| ### (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M
| | ##7720μM*x mL=6mL*(1.5*120μM) |
| ### 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
| | ###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ### 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g | | ###5.266mL H<sub>2</sub>O |
| ### .0283g of the .0924g stuck to the weigh paper, leaving .0639g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]. | | ##7720μM*x mL=6mL*(1.5*128μM) |
| ### .0639g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | | ###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ### .000188mol/.025L=xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | | ###5.251mL H<sub>2</sub>O |
| ### .007520M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] | | ##7720μM*x mL=6mL*(1.5*130μM) |
| ### .0639g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution | | ###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ##A 15μM stock solution of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was made
| | ###5.248mL H<sub>2</sub>O |
| ### (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 | | ##7720μM*x mL=6mL*(1.5*132μM) |
| ### 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
| | ###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ### 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] | | ###5.246mL H<sub>2</sub>O |
| ### .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution | | ##7720μM*x mL=6mL*(1.5*133μM) |
| ##Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 120, 128, 130, 132, 133, and 134. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL.
| | ###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | | ###5.245mL H<sub>2</sub>O |
| ###15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6) | | ##7720μM*x mL=6mL*(1.5*134μM) |
| ####0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube
| | ###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ###7520μM*x mL=6mL*(1.5*60μM) | | ###5.254mL H<sub>2</sub>O |
| ####0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | ##7720μM*x mL=6mL*(1.5*136μM) |
| ####5.331 mL H<sub>2</sub>O
| | ###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| | | ###5.244mL H<sub>2</sub>O |
| ###7520μM*x mL=6mL*(1.5*120μM)
| | ##7720μM*x mL=6mL*(1.5*138μM) |
| ####0.143mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | ###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ####5.257mL H<sub>2</sub>O
| | ###5.242mL H<sub>2</sub>O |
| ###7520μM*x mL=6mL*(1.5*128μM) | | ##7720μM*x mL=6mL*(1.5*140μM) |
| ####0.153mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | ###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ####5.247mL H<sub>2</sub>O
| | ###5.237mL H<sub>2</sub>O |
| ###7520μM*x mL=6mL*(1.5*130μM) | | ##7720μM*x mL=6mL*(1.5*160μM) |
| ####.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | | ###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ####5.245mL H<sub>2</sub>O
| | ###5.213mL H<sub>2</sub>O |
| ###7520μM*x mL=6mL*(1.5*132μM) | | ##7720μM*x mL=6mL*(1.5*170μM) |
| ####0.157mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | ###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution |
| ####5.243mL H<sub>2</sub>O
| | ###5.202mL H<sub>2</sub>O |
| ###7520μM*x mL=6mL*(1.5*133μM) | |
| ####0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ####5.241mL H<sub>2</sub>O
| |
| ###7520μM*x mL=6mL*(1.5*134μM) | |
| ####0.160mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ####5.240mL H<sub>2</sub>O
| |
| | |
| #The tubes were wrapped in tin foil and heated at 80°C for 4 hours. | | #The tubes were wrapped in tin foil and heated at 80°C for 4 hours. |
| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Project name
