User:Michael F. Nagle/Notebook/Chem 571/2012/09/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objectives==
==Objective==
* Using UV/Vis, find which mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] result in the most [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] unfolding and formation of gold nanoparticles.
*Make solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] with varying mole ratios
* Make solutions with varying concentrations of Tris buffer and [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
==Procedure==
==Procedure==
#UV/Vis
#A stock solution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was made, with the attempted Molarity of 10mM.
##1mL of each solution with a varying mole ratios of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was inserted into a cuvette, which went into a UV/Vis Spectroscoper
## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M
##Spectra were obtained and peaks for gold nanoparticles were identified at the 520nm range.
## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
##Cuvette was cleaned between each spectra, and the same cuvette was used each time
## 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g
#Tris buffer serial dilution
## .0197g of the .0849g stuck to the weigh paper, leaving .0656g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]].
##A .01M stock solution of Tris was made by putting .0121g Tris in 10mL H<sub>2</sub>O
## .0656g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
### [(mass Tris)* 121.14g/mol Tris]/.01L = .01M Tris solution
## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]
### .0121g Tris
## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution
## 1mL was taken from the tube with a pipette and moved to the next tube. 1mL from this tube was moved to the next tube, and so on until all get some amount of Tris.
#A 15μM stock solution of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was made
## 9mL H<sub>2</sub>O was put in the first tube to receive 1mL Tris from the stock solution.
## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015
## A serial dilution was repeated with H<sub>2</sub>O rather than Tris.
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
## Test tubes were wrapped and heated at at 80°C for 4 hours.
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 19:39, 10 September 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Make solutions of HAuCl4 and BSA with varying mole ratios

Procedure

  1. A stock solution of HAuCl4 was made, with the attempted Molarity of 10mM.
    1. (moles HAuCl4)/.025L H2O = .01M
    2. 2.5*10-4 mol HAuCl4
    3. 2.5*10-4 mol * 339.785mol/g HAuCl4 = .0849g
    4. .0197g of the .0849g stuck to the weigh paper, leaving .0656gHAuCl4.
    5. .0656g HAuCl4 * (1molHAuCl4/339.79g/molHAuCl4)= xM HAuCl4
    6. .007721M HAuCl4
    7. .0849g HAuCl4 was put in 25mL to make a 10mM solution
  2. A 15μM stock solution of BSA was made
    1. (moles BSA)/.025L = .000015
    2. 3.75*10^-7mol BSA
    3. 3.75*10^-7mol BSA * 66,463g/mol BSA = .0249g BSA
    4. .0249g BSA was put in 25mL to make a 15μM solution


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04&oldid=624921"