User:Michael F. Nagle/Notebook/Chem 571/2012/08/29: Difference between revisions

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## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
==Results==
*The BSA solution created was 15μM
*The actual molarity for the HAuCl<sub>4</sub> stock solution created turned out to be 7.721mM rather than 10mM. This is because unlike BSA, which is easy to handle, HAuCl4 comes in large chunks which don't break easily and melts, leaving residue on the weigh paper.
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Revision as of 21:06, 13 September 2012

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Objective

Procedure

  1. A stock solution of HAuCl4 was made, with the attempted Molarity of 10mM.
    1. (moles HAuCl4)/.025L H2O = .01M
    2. 2.5*10-4 mol HAuCl4
    3. 2.5*10-4 mol * 339.785mol/g HAuCl4 = .0849g
    4. .0197g of the .0849g stuck to the weigh paper, leaving .0656gHAuCl4.
    5. .0656g HAuCl4 * (1molHAuCl4/339.79g/molHAuCl4)= xM HAuCl4
    6. .007721M HAuCl4
    7. .0849g HAuCl4 was put in 25mL to make a 10mM solution
  2. A 15μM stock solution of BSA was made
    1. (moles BSA)/.025L = .000015
    2. 3.75*10^-7mol BSA
    3. 3.75*10^-7mol BSA * 66,463g/mol BSA = .0249g BSA
    4. .0249g BSA was put in 25mL to make a 15μM solution

Results

  • The BSA solution created was 15μM
  • The actual molarity for the HAuCl4 stock solution created turned out to be 7.721mM rather than 10mM. This is because unlike BSA, which is easy to handle, HAuCl4 comes in large chunks which don't break easily and melts, leaving residue on the weigh paper.


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