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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Objective== | | ==Objective== |
| *Find optimal mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] to [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] for formation of gold nanoparticles | | *Make stock solutions of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] |
| ==Procedure== | | ==Procedure== |
| #Solutions were made with the mole ratios of ([[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/[[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]) 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution from 6mL. | | #A stock solution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was made, with the attempted Molarity of 10mM. |
| ##m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
| | ## (moles [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])/.025L H<sub>2</sub>O = .01M |
| ##15μM*(volume [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution) = (1.5*6)
| | ## 2.5*10<sup>-4</sup> mol [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] |
| ###0.6mL [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] stock solution in each tube | | ## 2.5*10<sup>-4</sup> mol * 339.785mol/g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] = .0849g |
| ##7720μM*x mL=6mL*(1.5*60μM)
| | ## .0197g of the .0849g stuck to the weigh paper, leaving .0656g[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]. |
| ###0.069mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | ## .0656g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] * (1mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]]/339.79g/mol[[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]])= xM [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] |
| ###5.331 mL H<sub>2</sub>O | | ## .007721M [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] |
| ##7720μM*x mL=6mL*(1.5*80μM)
| | ## .0849g [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] was put in 25mL to make a 10mM solution |
| ###0.093mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | #A 15μM stock solution of [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was made |
| ###5.307mL H<sub>2</sub>O | | ## (moles [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]])/.025L = .000015 |
| ##7720μM*x mL=6mL*(1.5*100μM)
| | ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] |
| ###0.117mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| | ## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] |
| ###5.283mL H<sub>2</sub>O
| | ## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution |
| ##7720μM*x mL=6mL*(1.5*120μM) | |
| ###0.134mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.266mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*128μM)
| |
| ###0.149mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.251mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*130μM)
| |
| ###.152mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.248mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*132μM)
| |
| ###0.154mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.246mL H<sub>2</sub>O | |
| ##7720μM*x mL=6mL*(1.5*133μM)
| |
| ###0.155mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.245mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*134μM) | |
| ###0.156mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.254mL H<sub>2</sub>O | |
| ##7720μM*x mL=6mL*(1.5*136μM) | |
| ###0.159mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.244mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*138μM)
| |
| ###0.161mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution | |
| ###5.242mL H<sub>2</sub>O | |
| ##7720μM*x mL=6mL*(1.5*140μM)
| |
| ###0.163mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.237mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*160μM)
| |
| ###0.187mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.213mL H<sub>2</sub>O
| |
| ##7720μM*x mL=6mL*(1.5*170μM)
| |
| ###0.198mL [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] stock solution
| |
| ###5.202mL H<sub>2</sub>O | |
| #The tubes were wrapped in tin foil and heated at 80°C for 4 hours.
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| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
| |} | | |} |
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| __NOTOC__ | | __NOTOC__ |