User:MichaelMoore

I am a second year graduate student in the BBS program at Harvard Med and one of the Silver lab's newest members. A major focus in Pam's lab is the system-wide analysis of RNA dynamics in yeast and mammalian cells. To that end, several past and present Silver lab members have pioneered the use of chromatin immunoprecipitation followed by DNA microarray analysis (ChIP on chip) to examine the coupling of gene expression to nuclear organization and various RNA processing events. I am working with Michael Yu to characterize genome-wide recuitment patterns of several core and auxiliary RNA splicing factors in yeast. It is our hope to understand the specificity of the major components of the splicing machinery, to correlate that data to factors such as expression levels and/or gene structure, and ultimately to extend out findings to mammalian systems, where the biochemical mechanism of splicing and many of the relevant RNA and protein effectors are highly conserved.