User:Meshael Abusalem/Notebook/Biology 210 at AU

Lab Notebook Entry #5: Plants and Fungus

February 12, 2016

Location of plant samples with transect:

The plants were dispersed across the transect and somewhat difficult to find due to the fact that most of the leaves this time of year are dead. The plants that were collected and examined were found near the water ditch. This could possibly allow someone to hypothesize that the plants that were able to survive were those that were able to be nurtured through the water ditch.

Plant Characterization Table

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Lab Notebook Entry #4: Microbiology and Bacteria

February 5, 2016

Serial Dilution Results Table

Colony Characteristics

Materials and Methods

Gram Stain: The flamed loop was heated then used to take a bacteria culture from the bacteria plate. The culture was put on a slide along with a drop of water. The culture and water droplet were heated over a bunsen burner in order to remove the water. The culture was covered with crystal violet and stood for 30 seconds then rinsed with water. This process of adding a solution then waiting for 30 seconds was repeated with iodine and safranin stain as well. The culture was rinsed with alcohol and stood for 10-20 seconds after the iodine solution was added. Between each solution the culture was rinsed with water.

DNA Isolation and PCR Amplification: PCR tubes were labeled with transect number and colony number. The tubes were filled with a 20ul of primer/water mixture, then lightly shaken to ensure the mixture was dissolved. A small toothpick was used pick up a small amount of the bacteria culture. The toothpick was used to mix the culture in the primer/water. The PCR tube was covered and placed into the PCR machine.

Colony Morphology and Gram Strain

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Lab Notebook Entry #1: Examining Biological Life at AU