User:Meshael Abusalem/Notebook/Biology 210 at AU

Lab Notebook Entry #4: Microbiology and Bacteria

Serial Dilution Results Table

Colony Characteristics

Materials and Methods

Gram Stain: The flamed loop was heated then used to take a bacteria culture from the bacteria plate. The culture was put on a slide along with a drop of water. The culture and water droplet were heated over a bunsen burner in order to remove the water. The culture was covered with crystal violet and stood for 30 seconds then rinsed with water. This process of adding a solution then waiting for 30 seconds was repeated with iodine and safranin stain as well. The culture was rinsed with alcohol and stood for 10-20 seconds after the iodine solution was added. Between each solution the culture was rinsed with water.

DNA Isolation and PCR Amplification: PCR tubes were labeled with transect number and colony number. The tubes were filled with a 20ul of primer/water mixture, then lightly shaken to ensure the mixture was dissolved. A small toothpick was used pick up a small amount of the bacteria culture. The toothpick was used to mix the culture in the primer/water. The PCR tube was covered and placed into the PCR machine.

Colony Morphology and Gram Strain

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Lab Notebook Entry #1: Examining Biological Life at AU