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| =Lab Notebook Entry #4: Microbiology and Bacteria=
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| == Serial Dilution Results Table ==
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| [[Image:Screen Shot 2016-02-04 at 10.39.44 PM.png]]
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| == Colony Characteristics ==
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| [[Image:Screen_Shot_2016-02-05_at_11.03.57_AM.png]]
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| == Materials and Methods ==
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| Gram Stain:
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| The flamed loop was heated then used to take a bacteria culture from the bacteria plate. The culture was put on a slide along with a drop of water. The culture and water droplet were heated over a bunsen burner in order to remove the water. The culture was covered with crystal violet and stood for 30 seconds then rinsed with water. This process of adding a solution then waiting for 30 seconds was repeated with iodine and safranin stain as well. The culture was rinsed with alcohol and stood for 10-20 seconds after the iodine solution was added. Between each solution the culture was rinsed with water.
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| DNA Isolation and PCR Amplification:
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| PCR tubes were labeled with transect number and colony number. The tubes were filled with a 20ul of primer/water mixture, then lightly shaken to ensure the mixture was dissolved. A small toothpick was used pick up a small amount of the bacteria culture. The toothpick was used to mix the culture in the primer/water. The PCR tube was covered and placed into the PCR machine.
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| == Colony Morphology and Gram Strain==
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| [[Image:IMG_1036.JPG]]
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| [[Image:IMG_1030.JPG]]
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| [[Image:IMG_1026.JPG]]
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| [[Image:IMG_1021.JPG]]
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| ----
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| =Lab Notebook Entry #1: Examining Biological Life at AU=
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| [[Image:Transect2.jpg]]
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