User:Meredith Gleason/Notebook/Lab 1: Transect Observation/Hay Infusion Culture (7/1/14)/Entry Base: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreated Lab Notebook name=User:Meredith Gleason/Notebook/Lab 1: Transect Observation/Hay Infusion Culture (7/1/14)/Entry Base, content from MediaWiki:EntryContentDefault)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
'''Date: 7/1/14'''
* Insert content here...


'''Objective:'''This project focuses on a transect of land near the campus of American University in Washington, DC. While most would consider the campus to be an "urban environment", there is in fact quite a bit of green space available to analyze the ecosystems that exist on campus.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
'''Methods/Observations:''' I traveled to the site of transect, Site #2, and made preliminary observations about the site. Site #2 is located on the grounds of the Wesley Theological Seminary, located just outside the northwestern boundaries of American University's campus. The transect of land, approximately 20x20 feet in size, is a wooded area with 6 pine trees with a dense area of ground cover below. Of the 6 trees, 3 are coniferous and 3 are deciduous. This transect is surrounded on all sides by grass but within the confines of the ~20x20 area of the trees, the ground is not covered with grass, but is instead covered with pine needles that have fallen and various short ground plants, like ivies and common weeds. An aerial view of the area is seen here:
|}


__NOTOC__
[[Image:aerial_view_of_transect_site_2.png]]
 
 
And an eye-level photo is seen here:
 
[[Image:MG_TransectSite2Photo - eyeview.jpg]]
 
At the site, an 11.6 g sample was taken, including soil found ~10 feet between two coniferous trees, along with pine needles and small leaves of ground ferns and ivies growing in the soil.  Upon return to the lab, a Hay Infusion Culture was made with the entire soil sample, 0.1 g of dried milk, and 500 mL of filtered, Deerpark water in a large jar. The sample was vigorously mixed and left - with the lid open - on a lab bench to slowly separate.
 
'''Results & Conclusions:''' The result was, primarily, to make observations and set up a Hay Infusion Culture to be able to identify protists at a future date. Conclusions at this point are not appropriate as the infusion culture has not been studied.

Latest revision as of 00:05, 27 September 2017

Project name Main project page

Date: 7/1/14

Objective:This project focuses on a transect of land near the campus of American University in Washington, DC. While most would consider the campus to be an "urban environment", there is in fact quite a bit of green space available to analyze the ecosystems that exist on campus.

Methods/Observations: I traveled to the site of transect, Site #2, and made preliminary observations about the site. Site #2 is located on the grounds of the Wesley Theological Seminary, located just outside the northwestern boundaries of American University's campus. The transect of land, approximately 20x20 feet in size, is a wooded area with 6 pine trees with a dense area of ground cover below. Of the 6 trees, 3 are coniferous and 3 are deciduous. This transect is surrounded on all sides by grass but within the confines of the ~20x20 area of the trees, the ground is not covered with grass, but is instead covered with pine needles that have fallen and various short ground plants, like ivies and common weeds. An aerial view of the area is seen here:


And an eye-level photo is seen here:

At the site, an 11.6 g sample was taken, including soil found ~10 feet between two coniferous trees, along with pine needles and small leaves of ground ferns and ivies growing in the soil. Upon return to the lab, a Hay Infusion Culture was made with the entire soil sample, 0.1 g of dried milk, and 500 mL of filtered, Deerpark water in a large jar. The sample was vigorously mixed and left - with the lid open - on a lab bench to slowly separate.

Results & Conclusions: The result was, primarily, to make observations and set up a Hay Infusion Culture to be able to identify protists at a future date. Conclusions at this point are not appropriate as the infusion culture has not been studied.