User:Melissa Novy/Notebook/CHEM-581/2012/11/16

Objectives

• Standardize 1 M NaHCO₃ solution.
• Soak PVOH (MW 22000) in HPLC grade H₂O to determine change in mass due to swelling.
• Soak PVOH (MW 22000), 1 mL glutaraldehyde, 1 mL porphyrin in 2 wt% H₂SO₄.

Standardization of NaHCO₃

• Protocol
  1. Weigh out 20 g of potassium hydrogen phthalate (KHP).
  2. The mass was divided into thirds, and then dried in the oven at 90°C for 48 hours.
  3. Each sample was divided in half again to give six samples of KHP total.
  4. One sample was placed in a 100 mL beaker and 2 drops of bromothymol blue was added.
  5. 1 M NaHCO₃ was poured into a titration buret and the initial volume was recorded.
  6. The KHP was titrated with the NaHCO₃ solution until the solution in the beaker turned blue and stopped fizzing.
  7. The process was repeated for two more KHP samples.
  8. The number of moles in the NaHCO₃ solution was calculated.

CALCULATIONS