User:Melissa Novy/Notebook/CHEM-572/2013/04/16

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(Autocreate 2013/04/16 Entry for User:Melissa_Novy/Notebook/CHEM-572)
Current revision (11:56, 22 April 2013) (view source)
(Entry title)
 
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==Entry title==
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==Objectives==
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* Insert content here...
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* Begin 8-day study of films on silver leaching and antibacterial effects.
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** Note that all films were cut into 0.5-gram pieces before being tested.
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==Eight-Day Silver Leaching Study==
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* The protocol on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/20|2013/02/20]] was followed, except that solutions were placed in polypropylene beakers to prevent silver adsorption.
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* Poly(lactic acid) films containing 33wt% [[User:Melissa_Novy/Notebook/CHEM-572/2013/04/10|105AgLMT]] or [[User:Melissa_Novy/Notebook/CHEM-572/2013/04/10|33wt% LMT + AgNO<sub>3</sub>]] were tested.
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* Measurements will be taken once per day over a period of 8 days.
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==Eight-Day Antibacterial Effect Study with <i>E. coli</i>==
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* A protocol similar to that used in the 15-hour study on [[User:Melissa_Novy/Notebook/CHEM-572/2013/04/07|2013/04/07]] was followed.
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* Films made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/04/10|2013/04/10]] (PLA2002D + 33wt% 105AgLMT and PLA2002D + 33wt% LMT + AgNO<sub>3</sub>) were used.
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* Films were left to soak in autoclaved LB media for a period of 1, 3, 5 or 7 days.  Subsequently, 1 mL of <i>E. coli</i> cells was added to the films in LB and shaken in an orbital shaker at 230 rpm and 37°C for 24 h.  The absorbance of the solutions was observed at 600 nm at the end of the 24-h period to determine the extent of bacterial growth.
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* Please refer to Keyun Wang's [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/04/16|entry]] for more information.
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Objectives

  • Begin 8-day study of films on silver leaching and antibacterial effects.
    • Note that all films were cut into 0.5-gram pieces before being tested.

Eight-Day Silver Leaching Study

  • The protocol on 2013/02/20 was followed, except that solutions were placed in polypropylene beakers to prevent silver adsorption.
  • Poly(lactic acid) films containing 33wt% 105AgLMT or 33wt% LMT + AgNO3 were tested.
  • Measurements will be taken once per day over a period of 8 days.

Eight-Day Antibacterial Effect Study with E. coli

  • A protocol similar to that used in the 15-hour study on 2013/04/07 was followed.
  • Films made on 2013/04/10 (PLA2002D + 33wt% 105AgLMT and PLA2002D + 33wt% LMT + AgNO3) were used.
  • Films were left to soak in autoclaved LB media for a period of 1, 3, 5 or 7 days. Subsequently, 1 mL of E. coli cells was added to the films in LB and shaken in an orbital shaker at 230 rpm and 37°C for 24 h. The absorbance of the solutions was observed at 600 nm at the end of the 24-h period to determine the extent of bacterial growth.
  • Please refer to Keyun Wang's entry for more information.



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