User:Melissa Novy/Notebook/CHEM-572/2013/04/07

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(Autocreate 2013/04/07 Entry for User:Melissa_Novy/Notebook/CHEM-572)
Current revision (17:12, 10 April 2013) (view source)
(Cell Growth Study)
 
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==Entry title==
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==Objectives==
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* Insert content here...
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* Conduct 15-hour cell growth study with antimicrobial films.
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==Cell Growth Study==
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* Films made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/04/03|2013/04/03]] were used.
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* Please refer to the protocol in Keyun Wang's [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/04/07|entry]].  Note that the mass of each film placed in each flask was 0.5 g.
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* Based on the measured absorbances of each solution, it must be concluded that there was no difference in antimicrobial activity between the control films and the films containing silver-loaded Laponite.
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Hour'''
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| align="center" style="background:#f0f0f0;"|'''Absorbance []'''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| ||Inoculated LB and PLA2002D + 33wt% LMT Average||Standard Deviation||Inoculated LB and PLA2002D + 17wt% 100AgLMT Average||Standard Deviation
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|-
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| 0||0.002219443||0.007688375||0.044696002||0.062617099
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|-
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| 3||0.002364866||0.008192137||0.128268254||0.080308048
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|-
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| 6||0.011864981||0.0411015||0.525183657||0.558716009
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|-
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| 9||0.023904904||0.082809017||0.689716945||1.102695472
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|-
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| 12||0.014237405||0.049319818||0.733402454||1.213819081
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|-
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| 15||0.012357334||0.042807061||0.745480877||1.244062054
|}
|}
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<br>
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[[Image:04-07-2013.jpg]]
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Objectives

  • Conduct 15-hour cell growth study with antimicrobial films.

Cell Growth Study

  • Films made on 2013/04/03 were used.
  • Please refer to the protocol in Keyun Wang's entry. Note that the mass of each film placed in each flask was 0.5 g.
  • Based on the measured absorbances of each solution, it must be concluded that there was no difference in antimicrobial activity between the control films and the films containing silver-loaded Laponite.


Hour Absorbance [] ' ' '
Inoculated LB and PLA2002D + 33wt% LMT AverageStandard DeviationInoculated LB and PLA2002D + 17wt% 100AgLMT AverageStandard Deviation
00.0022194430.0076883750.0446960020.062617099
30.0023648660.0081921370.1282682540.080308048
60.0118649810.04110150.5251836570.558716009
90.0239049040.0828090170.6897169451.102695472
120.0142374050.0493198180.7334024541.213819081
150.0123573340.0428070610.7454808771.244062054


Image:04-07-2013.jpg



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