User:Melissa Novy/Notebook/CHEM-572/2013/03/05

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Objectives

  • Make 16 separate 50-mL portions of LB media in 250 mL Erlenmeyer flasks.
  • Autoclave the 16 solutions of LB media and three boxes of 1000 μL pipet tips.
  • Make a 3-gram film of PLA2002D + 10 wt% 100Ag-LMT.

LB Media

  • Sixteen 250-mL Erlenmeyer flasks were washed and dried.
  • Then, 1.25 g of LB media, obtained from Teknova, and 50 mL of deionized water was added to each flask.
  • The flasks were capped with foil and autoclaved, according to the usual procedure.

PLA2002D + 10 wt% 100Ag-LMT Film

  • Theoretical mass PLA2002D: 3.000 g
  • Theoretical mass 100Ag-LMT: 0.30 g


  • Actual mass PLA2002D: 3.00600 g
  • Actual mass 100Ag-LMT: 0.30430 g


  • 100Ag-LMT made on 2013/02/05 was used.
  • Please refer to the entry on 2013/02/06 for the protocol used to make the films.



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