User:Melissa Novy/Notebook/CHEM-572/2013/02/19

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(→PLA2002D Films)
Current revision (11:00, 27 February 2013) (view source)
(→Objectives)
 
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* Make PLA2002D + 5 wt% 100Ag-LMT film.
* Make PLA2002D + 5 wt% 100Ag-LMT film.
* Make PLA2002D + 5 wt% LMT film.
* Make PLA2002D + 5 wt% LMT film.
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* Prepare five starter cultures of DH5α-T1 cells and autoclave LB media for Ag<sup>+</sup> cell growth inhibition studies.
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==Starter Cultures==
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* Protocol
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*# Place 3 mL of sterile LB media into a 15-mL polypropylene Falcon tube.
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*# Using a sterile wooden stick, inoculate one colony of DH5α-T1 cells and dip the stick into the LB media.
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*# Repeat steps 1 and 2 four more times, to obtain 5 Falcon tubes total.
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*# Place the Falcon tubes in an orbital shaker at 230 rpm and 37°C for 15 hours.
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==Preparation of LB Media==
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* Six LB media solutions of 50 mL each were prepared.  Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/02/19|Keyun Wang's entry]] for the protocol and masses needed of the LB media components.
==PLA2002D Films==
==PLA2002D Films==

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Objectives

  • Grind 100Ag-LMT made on 2013/02/05 into a fine powder.
  • Make PLA2002D + 5 wt% 100Ag-LMT film.
  • Make PLA2002D + 5 wt% LMT film.
  • Prepare five starter cultures of DH5α-T1 cells and autoclave LB media for Ag+ cell growth inhibition studies.

Starter Cultures

  • Protocol
    1. Place 3 mL of sterile LB media into a 15-mL polypropylene Falcon tube.
    2. Using a sterile wooden stick, inoculate one colony of DH5α-T1 cells and dip the stick into the LB media.
    3. Repeat steps 1 and 2 four more times, to obtain 5 Falcon tubes total.
    4. Place the Falcon tubes in an orbital shaker at 230 rpm and 37°C for 15 hours.


Preparation of LB Media

  • Six LB media solutions of 50 mL each were prepared. Please refer to Keyun Wang's entry for the protocol and masses needed of the LB media components.

PLA2002D Films

  • The protocol on 2013/02/06 was used to make the films.
  • Each film contained 5 wt% of 100Ag-LMT or LMT for one gram of PLA2002D.


  • PLA2002D + 5 wt% LMT film
    • Theoretical mass PLA2002D: 1 g
    • Theoretical mass LMT: 0.05 g
    • Actual mass PLA2002D: 1.009185 g
    • Actual mass LMT: 0.05060 g


  • PLA2002D + 5 wt% 100Ag-LMT film
    • Theoretical mass PLA2002D: 1 g
    • Theoretical mass 100Ag-LMT: 0.05 g
    • Actual mass PLA2002D: 1.01686 g
    • Actual mass 100Ag-LMT: 0.05062 g
    • Final mass of PLA2002D + 5 wt% 100Ag-LMT: 0.97 g


  • Actual content of silver in 0.5 g PLA2002D + 5 wt% 100Ag-LMT: 


  0.00622 g AgNO3 × (1 mol AgNO3/169.87 g AgNO3) × (1 mol Ag+/1 mol AgNO3) = 3.66 × 10-5 mol Ag+
  [Ag+] in 5 mL 100Ag-LMT filtrate was 0.000253614 M
  0.000253614 M Ag+ × 0.005 L = 1.2681 × 10-6 mol Ag+
  Therefore, 3.66 × 10-5 mol Ag+ − 1.2681 × 10-6 mol Ag+ = 3.53 × 10-5 mol Ag+ was exchanged into 100Ag-LMT.



  0.05 × 0.5 g PLA2002D + 5 wt% 100Ag-LMT = 0.025 g 100Ag-LMT
  (3.53 × 10-5 mol Ag+) ÷ (0.05062 g 100Ag-LMT) = x mol Ag+ ÷ (0.025 g 100Ag-LMT)
  4.47 × 10-8mol Ag+ in 0.025 g 100Ag-LMT of 0.5 g PLA2002D + 5 wt% 100Ag-LMT film



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