User:Melissa Novy/Notebook/CHEM-572/2013/02/19

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==Objectives==
==Objectives==
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* Grind 100Ag-LMT made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/05|2013/02/05]] into a fine powder.
* Make PLA2002D + 5 wt% 100Ag-LMT film.
* Make PLA2002D + 5 wt% 100Ag-LMT film.
* Make PLA2002D + 5 wt% LMT film.
* Make PLA2002D + 5 wt% LMT film.
 +
* Prepare five starter cultures of DH5α-T1 cells and autoclave LB media for Ag<sup>+</sup> cell growth inhibition studies.
 +
 +
==Starter Cultures==
 +
* Protocol
 +
*# Place 3 mL of sterile LB media into a 15-mL polypropylene Falcon tube.
 +
*# Using a sterile wooden stick, inoculate one colony of DH5α-T1 cells and dip the stick into the LB media.
 +
*# Repeat steps 1 and 2 four more times, to obtain 5 Falcon tubes total.
 +
*# Place the Falcon tubes in an orbital shaker at 230 rpm and 37°C for 15 hours.
 +
 +
 +
==Preparation of LB Media==
 +
* Six LB media solutions of 50 mL each were prepared.  Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/02/19|Keyun Wang's entry]] for the protocol and masses needed of the LB media components.
 +
 +
==PLA2002D Films==
 +
* The protocol on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/06|2013/02/06]] was used to make the films.
 +
* Each film contained 5 wt% of 100Ag-LMT or LMT for one gram of PLA2002D.
 +
<br>
 +
 +
* PLA2002D + 5 wt% LMT film
 +
** Theoretical mass PLA2002D: 1 g
 +
** Theoretical mass LMT: 0.05 g
 +
** Actual mass PLA2002D: 1.009185 g
 +
** Actual mass LMT: 0.05060 g
 +
<br>
 +
 +
* PLA2002D + 5 wt% [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/05|100Ag-LMT]] film
 +
** Theoretical mass PLA2002D: 1 g
 +
** Theoretical mass 100Ag-LMT: 0.05 g
 +
** Actual mass PLA2002D: 1.01686 g
 +
** Actual mass 100Ag-LMT: 0.05062 g
 +
** Final mass of PLA2002D + 5 wt% 100Ag-LMT: 0.97 g
 +
<br>
 +
 +
* Actual content of silver in 0.5 g PLA2002D + 5 wt% 100Ag-LMT:
 +
** Please refer to the entries on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/05|2013/02/05]] and [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/13|2013/02/13]] for the data listed below.
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<br>
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 +
  '''0.00622 g AgNO<sub>3</sub> × (1 mol AgNO<sub>3</sub>/169.87 g AgNO<sub>3</sub>) × (1 mol Ag<sup>+</sup>/1 mol AgNO<sub>3</sub>) = 3.66 × 10<sup>-5</sup> mol Ag<sup>+</sup>'''
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  '''[Ag<sup>+</sup>] in 5 mL 100Ag-LMT filtrate was 0.000253614 M'''
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  '''0.000253614 M Ag<sup>+</sup> × 0.005 L = 1.2681 × 10<sup>-6</sup> mol Ag<sup>+</sup>'''
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  '''Therefore, 3.66 × 10<sup>-5</sup> mol Ag<sup>+</sup> − 1.2681 × 10<sup>-6</sup> mol Ag<sup>+</sup> = 3.53 × 10<sup>-5</sup> mol Ag<sup>+</sup> was exchanged into 100Ag-LMT.'''
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<br>
 +
 +
  '''0.05 × 0.5 g PLA2002D + 5 wt% 100Ag-LMT = 0.025 g 100Ag-LMT
 +
  '''(3.53 × 10<sup>-5</sup> mol Ag<sup>+</sup>) ÷ (0.05062 g 100Ag-LMT) = x mol Ag<sup>+</sup> ÷ (0.025 g 100Ag-LMT)'''
 +
  '''4.47 × 10<sup>-8</sup>mol Ag<sup>+</sup> in 0.025 g 100Ag-LMT of 0.5 g PLA2002D + 5 wt% 100Ag-LMT film'''
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Objectives

  • Grind 100Ag-LMT made on 2013/02/05 into a fine powder.
  • Make PLA2002D + 5 wt% 100Ag-LMT film.
  • Make PLA2002D + 5 wt% LMT film.
  • Prepare five starter cultures of DH5α-T1 cells and autoclave LB media for Ag+ cell growth inhibition studies.

Starter Cultures

  • Protocol
    1. Place 3 mL of sterile LB media into a 15-mL polypropylene Falcon tube.
    2. Using a sterile wooden stick, inoculate one colony of DH5α-T1 cells and dip the stick into the LB media.
    3. Repeat steps 1 and 2 four more times, to obtain 5 Falcon tubes total.
    4. Place the Falcon tubes in an orbital shaker at 230 rpm and 37°C for 15 hours.


Preparation of LB Media

  • Six LB media solutions of 50 mL each were prepared. Please refer to Keyun Wang's entry for the protocol and masses needed of the LB media components.

PLA2002D Films

  • The protocol on 2013/02/06 was used to make the films.
  • Each film contained 5 wt% of 100Ag-LMT or LMT for one gram of PLA2002D.


  • PLA2002D + 5 wt% LMT film
    • Theoretical mass PLA2002D: 1 g
    • Theoretical mass LMT: 0.05 g
    • Actual mass PLA2002D: 1.009185 g
    • Actual mass LMT: 0.05060 g


  • PLA2002D + 5 wt% 100Ag-LMT film
    • Theoretical mass PLA2002D: 1 g
    • Theoretical mass 100Ag-LMT: 0.05 g
    • Actual mass PLA2002D: 1.01686 g
    • Actual mass 100Ag-LMT: 0.05062 g
    • Final mass of PLA2002D + 5 wt% 100Ag-LMT: 0.97 g


  • Actual content of silver in 0.5 g PLA2002D + 5 wt% 100Ag-LMT:


  0.00622 g AgNO3 × (1 mol AgNO3/169.87 g AgNO3) × (1 mol Ag+/1 mol AgNO3) = 3.66 × 10-5 mol Ag+
  [Ag+] in 5 mL 100Ag-LMT filtrate was 0.000253614 M
  0.000253614 M Ag+ × 0.005 L = 1.2681 × 10-6 mol Ag+
  Therefore, 3.66 × 10-5 mol Ag+ − 1.2681 × 10-6 mol Ag+ = 3.53 × 10-5 mol Ag+ was exchanged into 100Ag-LMT.


  0.05 × 0.5 g PLA2002D + 5 wt% 100Ag-LMT = 0.025 g 100Ag-LMT
  (3.53 × 10-5 mol Ag+) ÷ (0.05062 g 100Ag-LMT) = x mol Ag+ ÷ (0.025 g 100Ag-LMT)
  4.47 × 10-8mol Ag+ in 0.025 g 100Ag-LMT of 0.5 g PLA2002D + 5 wt% 100Ag-LMT film



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