User:Melissa Novy/Notebook/CHEM-572/2013/02/13

Objectives

• Make a calibration curve for the determination of Ag⁺ in solutions.
  • Make 0.10 M AgNO₃ solution.
• Remove PLA2002D + 5 wt % LMT film from glass mold.
• Prepare a starter culture of DH5α-T1 cells.
  • Please refer to Keyun Wang's entry for the protocol.

0.1 M AgNO₃ Solution

• Calculations

  250 mL of 0.1 M AgNO3
  MW AgNO3 = 169.87 g/mol
  0.1 M × 0.250 L = 0.025 mol
  0.025 mol AgNO3 × (169.87 g AgNO3/1 mol AgNO3) = 4.24675 g AgNO3

• Actual mass AgNO₃: 4.24694 g
• Actual molarity AgNO₃: 0.10000 M AgNO₃

Ag⁺ Ion Selective Electrode Calibration

• The protocol from 2013/02/12 was followed using solutions of the following [AgNO₃]:
  • 0 M AgNO₃
  • 0.0001 M AgNO₃
  • 0.0010 M AgNO₃
  • 0.0105 M AgNO₃
  • 0.1000M AgNO₃

• Conductivity of solutions of AgNO₃

• The error bars indicate a 5% deviation.

• Conductivity of filtrate solutions