# User:Melissa Novy/Notebook/CHEM-572/2013/02/13

(Difference between revisions)
 Revision as of 16:33, 19 February 2013 (view source) (→Ag+ Ion Selective Electrode Calibration)← Previous diff Current revision (11:02, 27 February 2013) (view source) (→Objectives) Line 19: Line 19: ** Make 0.10 M AgNO3 solution. ** Make 0.10 M AgNO3 solution. * Remove PLA2002D + 5 wt % LMT film from glass mold. * Remove PLA2002D + 5 wt % LMT film from glass mold. + * Prepare a starter culture of DH5α-T1 cells. + ** Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/02/13|Keyun Wang's entry]] for the protocol. ==0.1 M AgNO3 Solution== ==0.1 M AgNO3 Solution==

## Current revision

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## Objectives

• Make a calibration curve for the determination of Ag+ in solutions.
• Make 0.10 M AgNO3 solution.
• Remove PLA2002D + 5 wt % LMT film from glass mold.
• Prepare a starter culture of DH5α-T1 cells.

## 0.1 M AgNO3 Solution

• Calculations
```  250 mL of 0.1 M AgNO3
MW AgNO3 = 169.87 g/mol
0.1 M × 0.250 L = 0.025 mol
0.025 mol AgNO3 × (169.87 g AgNO3/1 mol AgNO3) = 4.24675 g AgNO3
```
• Actual mass AgNO3: 4.24694 g
• Actual molarity AgNO3: 0.10000 M AgNO3

## Ag+ Ion Selective Electrode Calibration

• The protocol from 2013/02/12 was followed using solutions of the following [AgNO3]:
• 0 M AgNO3
• 0.0001 M AgNO3
• 0.0010 M AgNO3
• 0.0105 M AgNO3
• 0.1000M AgNO3
• Conductivity of solutions of AgNO3
 [Ag+] [M] log [Ag+] [] Conductivity [mV] 0.00000 0 394.6 0.00000 0 390.7 0.00000 0 386.4 0.00010 -4 377 0.00010 -4 375.2 0.00010 -4 374.7 0.00100 -3 411.6 0.00100 -3 411.8 0.00100 -3 411.8 0.01050 -1.98 470.1 0.01050 -1.98 470.3 0.01050 -1.98 470.3 0.10000 -1 528.5 0.10000 -1 528.5 0.10000 -1 528.5

• The error bars indicate a 5% deviation.

• Conductivity of filtrate solutions
 Sample [] Conductivity [mV] Average [mV] log [Ag+] [] [Ag+] [M] (50:50) H2O/EtOH 372 374.3333333 -3.894305004 0.000127554 377.8 373.2 100Ag-LMT Filtrate 403.6 389.7333333 -3.595826469 0.000253614 389.3 376.3 95Ag-LMT Filtrate 582.3 582.7666667 0.145492134 1.397951596 582.9 583.1