User:Melissa Novy/Notebook/CHEM-572/2013/01/30

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Objectives

  • Make 6 sets of 50-mL LB media.
  • Make 500 mL agar solution.
  • Autoclave LB media and agar solution.
  • Make 20 agar plates.
  • Filter and dry LMT-95Ag, made on 2013/01/29.

LB Media

  • Protocol
    1. Obtain 6 clean, dry 250-mL Erlenmeyer flasks.
    2. Place 1.25 g of LB medium, obtained from Teknova, into each flask.
    3. Fill each flask with 50 mL deionized H2O.
    4. Cap each flask with aluminum foil and swirl until the LB dissolves.
    5. Autoclave the flasks according to the AU Biomaterials Design Lab protocol.

Agar Solution and Agar Plates

  • Protocol
    1. Obtain a clean, dry 1000-mL Erlenmeyer flask.
    2. Place 12.5 g of LB medium, obtained from Teknova, into the flask.
    3. Fill the flask with 500 mL deionized H2O.
    4. Swirl the flask to dissolve the LB, then add 7.5 g Trypticase Soy Agar, obtained from Aldrich.
    5. Cap the flask with aluminum foil and autoclave according to the AU Biomaterials Design Lab protocol.
    6. Obtain 20 sterile 10-cm Petri dishes.
    7. Fill each Petri dish with 25 mL of autoclaved agar solution.

Filter and Dry LMT-95Ag

  • LMT-95Ag was covered with aluminum foil and left to stir for 24 h. It was then filtered with Whatman 41 filter paper and a clean, dry filter flask and funnel. It was washed with 10 mL of (50:50) H2O-EtOH.
  • LMT-95Ag was then scraped from the filter paper with a spatula and placed in an aluminum dish loosely capped with foil in an 80°C oven to dry overnight.
  • The filtrate was reserved for further testing for sodium.
  • Observations
    • LMT-95Ag turned from opaque white in color to opaque dark gray. This indicates possible oxidation of Ag.
    • The filtrate was clear and colorless, but became transparent red-gray in color after about 30 min. The filtrate was subsequently covered in foil.


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