User:Melissa Novy/Notebook/CHEM-572/2013/01/30 - Revision history

Melissa Novy: /* Filter and Dry LMT-95Ag */

2013-02-05T18:26:20Z

Filter and Dry LMT-95Ag

←Older revision		Revision as of 18:26, 5 February 2013
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	* Observations		* Observations
	** LMT-95Ag turned from opaque white in color to opaque dark gray. This indicates possible oxidation of Ag.		** LMT-95Ag turned from opaque white in color to opaque dark gray. This indicates possible oxidation of Ag.
-	** The filtrate was clear and colorless.	+	** The filtrate was clear and colorless, but became transparent red-gray in color after about 30 min. The filtrate was subsequently covered in foil.

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Melissa Novy: /* Objectives */

2013-01-30T19:11:09Z

Objectives

←Older revision		Revision as of 19:11, 30 January 2013
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	* Make 500 mL agar solution.		* Make 500 mL agar solution.
	* Autoclave LB media and agar solution.		* Autoclave LB media and agar solution.
-	* ~~Pour~~ 20 agar plates.	+	* Make 20 agar plates.
	* Filter and dry LMT-95Ag, made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/29\|2013/01/29]].		* Filter and dry LMT-95Ag, made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/29\|2013/01/29]].

Melissa Novy at 19:06, 30 January 2013

2013-01-30T19:06:02Z

←Older revision		Revision as of 19:06, 30 January 2013
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-	==~~Entry title~~==	+	==Objectives==
-	* ~~Insert content here~~...	+	* Make 6 sets of 50-mL LB media.
		+	* Make 500 mL agar solution.
		+	* Autoclave LB media and agar solution.
		+	* Pour 20 agar plates.
		+	* Filter and dry LMT-95Ag, made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/29\|2013/01/29]].

		+	==LB Media==
		+	* Protocol
		+	*# Obtain 6 clean, dry 250-mL Erlenmeyer flasks.
		+	*# Place 1.25 g of LB medium, obtained from Teknova, into each flask.
		+	*# Fill each flask with 50 mL deionized H<sub>2</sub>O.
		+	*# Cap each flask with aluminum foil and swirl until the LB dissolves.
		+	*# Autoclave the flasks according to the [[AU_Biomaterials_Design_Lab:Protocols/Autoclave\|AU Biomaterials Design Lab protocol]].
		+
		+	* Note that the protocol was adapted from the [[LB\|OpenWetWare protocol]].
		+
		+	==Agar Solution and Agar Plates==
		+	* Protocol
		+	*# Obtain a clean, dry 1000-mL Erlenmeyer flask.
		+	*# Place 12.5 g of LB medium, obtained from Teknova, into the flask.
		+	*# Fill the flask with 500 mL deionized H<sub>2</sub>O.
		+	*# Swirl the flask to dissolve the LB, then add 7.5 g Trypticase Soy Agar, obtained from Aldrich.
		+	*# Cap the flask with aluminum foil and autoclave according to the [[AU_Biomaterials_Design_Lab:Protocols/Autoclave\|AU Biomaterials Design Lab protocol]].
		+	*# Obtain 20 sterile 10-cm Petri dishes.
		+	*# Fill each Petri dish with 25 mL of autoclaved agar solution.
		+
		+	==Filter and Dry LMT-95Ag==
		+	* LMT-95Ag was covered with aluminum foil and left to stir for 24 h. It was then filtered with Whatman 41 filter paper and a clean, dry filter flask and funnel. It was washed with 10 mL of (50:50) H<sub>2</sub>O-EtOH.
		+	* LMT-95Ag was then scraped from the filter paper with a spatula and placed in an aluminum dish loosely capped with foil in an 80°C oven to dry overnight.
		+	* The filtrate was reserved for further testing for sodium.
		+
		+	* Observations
		+	** LMT-95Ag turned from opaque white in color to opaque dark gray. This indicates possible oxidation of Ag.
		+	** The filtrate was clear and colorless.

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Melissa Novy: Autocreate 2013/01/30 Entry for User:Melissa_Novy/Notebook/CHEM-572

2013-01-30T18:49:56Z

Autocreate 2013/01/30 Entry for User:Melissa_Novy/Notebook/CHEM-572

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