User:Melissa Novy/Notebook/CHEM-571/2012/11/27

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Objectives

  • Analyze solutions of AuHRP and AuADA, both made on 2012/11/14 with UV-vis.
  • Make solutions of AuADA with dialyzed ADA.
    • ADA dialysis began on 2012/11/14 and ended on 2012/11/27.
    • Please refer to Keyun Wang's entry for the procedure used to remove the ADA from the dialysis tubing and prepare it for use in AuADA solutions.
  • Resuspend AuHRP fibers in Tris buffer.

UV-Vis on AuHRP and AuADA

  • Exactly 3 mL of each solution was pipetted into a quartz cuvette for analysis using an automated pipetter (Pipetman).
  • Please refer to Dhea Patel's entry for photographs of the solutions.
  • Observations:
    • Small pellets of fibers were visible in all AuHRP solutions except 60 AuHRP. The 60 AuHRP solution did not contain a fiber pellet. All AuHRP solutions were purple in color.
    • AuADA solutions were colorless and contained no aggregates.

Image:UV-Vis_Spectra_of_AuADA_11142012.JPG

  • The spectra for all solutions displayed a peak at around 520 nm, which indicates the presence of GNPs. The general trend was that the absorbance of these peaks increased as the mole ratio of Au:HRP increases. This implies that the amount of GNPs in solution increased in accordance with the amount of gold initially added to each solution.
  • However, the peak absorbances for the 130 and 140 AuHRP solutions were almost identical. This is likely because the 130 AuHRP solution contained 1 mL less of H2O due to an error during preparation of the solution.

Image:UV-Vis_Spectra_of_AuADA.JPG

Synthesizing AuADA Solutions

  • Please refer to the protocol on 2012/11/14. There were no deviations from the protocol and the same stock solution of HAuCl4 was used. However, dialyzed ADA was used instead of ADA in FPLC elution buffer.
  • Please refer to Keyun Wang's entry for observations made during the procedure.




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