User:Melissa Novy/Notebook/CHEM-571/2012/11/27

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==Entry title==
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==Objectives==
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* Insert content here...
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* Analyze solutions of AuHRP and AuADA, both made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/14|2012/11/14]] with UV-vis.
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* Make solutions of AuADA with dialyzed ADA.
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** ADA dialysis began on [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/14|2012/11/14]] and ended on 2012/11/27.
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==UV-Vis on AuHRP and AuADA==
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* Exactly 3 mL of each solution was pipetted into a quartz cuvette for analysis using an automated pipetter (Pipetman).
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* Please refer to Dhea Patel's entry for photographs of the solutions.
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* Observations:
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** Small pellets of fibers were visible in all AuHRP solutions except 60 AuHRP.  The 60 AuHRP solution did not contain a fiber pellet.  All AuHRP solutions were purple in color. 
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** AuADA solutions were colorless and contained no aggregates.
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==Synthesizing AuADA Solutions==
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* Please refer to the protocol on [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/14|2012/11/14]].  There were no deviations from the protocol and the same stock solution of HAuCl<sub>4</sub> was used.  However, dialyzed ADA was used instead of ADA in FPLC elution buffer.
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* Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/11/27|Keyun Wang's entry]] for observations made during the procedure.
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Revision as of 13:42, 27 November 2012

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Objectives

  • Analyze solutions of AuHRP and AuADA, both made on 2012/11/14 with UV-vis.
  • Make solutions of AuADA with dialyzed ADA.
    • ADA dialysis began on 2012/11/14 and ended on 2012/11/27.

UV-Vis on AuHRP and AuADA

  • Exactly 3 mL of each solution was pipetted into a quartz cuvette for analysis using an automated pipetter (Pipetman).
  • Please refer to Dhea Patel's entry for photographs of the solutions.
  • Observations:
    • Small pellets of fibers were visible in all AuHRP solutions except 60 AuHRP. The 60 AuHRP solution did not contain a fiber pellet. All AuHRP solutions were purple in color.
    • AuADA solutions were colorless and contained no aggregates.

Synthesizing AuADA Solutions

  • Please refer to the protocol on 2012/11/14. There were no deviations from the protocol and the same stock solution of HAuCl4 was used. However, dialyzed ADA was used instead of ADA in FPLC elution buffer.
  • Please refer to Keyun Wang's entry for observations made during the procedure.




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