User:Melissa Novy/Notebook/CHEM-571/2012/11/27

(Difference between revisions)

Revision as of 14:42, 27 November 2012

Analyze solutions of AuHRP and AuADA, both made on 2012/11/14 with UV-vis.
Make solutions of AuADA with dialyzed ADA.
- ADA dialysis began on 2012/11/14 and ended on 2012/11/27.

Exactly 3 mL of each solution was pipetted into a quartz cuvette for analysis using an automated pipetter (Pipetman).
Please refer to Dhea Patel's entry for photographs of the solutions.
Observations:
- Small pellets of fibers were visible in all AuHRP solutions except 60 AuHRP. The 60 AuHRP solution did not contain a fiber pellet. All AuHRP solutions were purple in color.
- AuADA solutions were colorless and contained no aggregates.

Please refer to the protocol on 2012/11/14. There were no deviations from the protocol and the same stock solution of HAuCl₄ was used. However, dialyzed ADA was used instead of ADA in FPLC elution buffer.
Please refer to Keyun Wang's entry for observations made during the procedure.

@@ Line 15: / Line 15: @@
 <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-==Entry title==
+==Objectives==
-* Insert content here...
+* Analyze solutions of AuHRP and AuADA, both made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/14|2012/11/14]] with UV-vis.
+* Make solutions of AuADA with dialyzed ADA.
+** ADA dialysis began on [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/14|2012/11/14]] and ended on 2012/11/27.
+==UV-Vis on AuHRP and AuADA==
+* Exactly 3 mL of each solution was pipetted into a quartz cuvette for analysis using an automated pipetter (Pipetman).
+* Please refer to Dhea Patel's entry for photographs of the solutions.
+* Observations:
+** Small pellets of fibers were visible in all AuHRP solutions except 60 AuHRP.  The 60 AuHRP solution did not contain a fiber pellet.  All AuHRP solutions were purple in color.
+** AuADA solutions were colorless and contained no aggregates.
+==Synthesizing AuADA Solutions==
+* Please refer to the protocol on [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/14|2012/11/14]].  There were no deviations from the protocol and the same stock solution of HAuCl<sub>4</sub> was used.  However, dialyzed ADA was used instead of ADA in FPLC elution buffer.
+* Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/11/27|Keyun Wang's entry]] for observations made during the procedure.