User:Melissa Novy/Notebook/CHEM-571/2012/11/13

Objectives

• Analyze Au-HRP solutions with UV-vis spectroscopy.
• Analyze solutions of Au-HRP and Au-lysozyme with AAS.
• Conduct ADA activity assay on ADA fractions using adenosine.

ADA Activity Assay

• 50 mL of 0.05 M sodium phosphate buffer was made with deionized water. The pH of the solution was adjusted to 7.4 using 3 drops of 12 M HCl.

• 5 mL of 0.1 mM adenosine was made.
  • 100 mg of adenosine was dissolved in 1 mL of 0.05 M phosphate buffer, giving a solution of 0.00373 M adenosine.
  • 0.134 mL of 0.00373 M adenosine was diluted to a final volume of 5 mL with 0.05 M phosphate buffer, giving a solution of 0.1 mM adenosine.

  (0.001 g)/(268.02 g/mol) = 3.73×10-6 mol
  (3.73×10-6 mol)/0.001 L = 0.00373 M
  (0.00373 M)(V1)=(5 mL)(0.1×10-3 M)
  V1 = 0.134 mL